Case Study: High-throughput SNP Screening Downstream of CRISPR/Cas9 Gene Editing
Posted 31st October 2018 by Kate Barlow
One of the most powerful applications of genome editing is the introduction of nucleotide substitutions in specific genomic sites. This can be used to mimic single-nucleotide polymorphisms (SNPs) or to generate stop codons that yield precise gene knockouts. However, screening hundreds of clones for a single edited nucleotide remains a challenge, especially in the absence of a corresponding phenotype.
Challenges of epigenetic studies in plants: focus on ChIP technique
Posted 26th October 2018 by Kate Barlow
Agnieszka Zelisko-Schmidt from Diagenode elaborates on their poster presented at the 6th Plant Genomics & Gene Editing Congress: Europe, explaining how regulatory pathways in plants can be unravelled using the universal plant ChIP-seq kit.
Increasing Shelf Life of Perishable Produce Using Patented Gene Technology
Posted 21st September 2018 by Kate Barlow
The eIF‑5A protein is highly conserved in all plants and animals and is the only protein in any organism that has the unique hypusine modification caused by the Deoxyhypusine Synthase (DHS) enzyme. The unmodified-to-hypusinated ratio of these two forms of eIF‑5A determines the fate of the cell. Recent studies suggest that eIF‑5A is mainly involved in RNA metabolism and movement through the cell, thereby regulating cell growth, proliferation, and programmed death. These stable genetic changes will significantly enhance crop traits, including:
An Updated Round-Up of the Top Plant Genome Databases
Posted 29th August 2018 by Kate Barlow
There’s more plant genome databases than there ever has been before. We’ve collated the most recent databases for ontology information on a variety of crops, including: rice, maize, wheat, sorghum, barley, millet, rye, and oats.
EU Court of Justice Rules that Gene Edited Organisms will be Regulated as GMOs
Posted 22nd August 2018 by Kate Barlow
On 25 July 2018, the Court of Justice of the European Union, the EU’s highest court, issued a decision clarifying whether the EU would regulate products of innovative breeding techniques, like gene editing, under the EU’s Directive 2001/18, the principal EU law governing the regulation of GMOs.
Transcriptomic Profiling of Pearl Millet for Salinity Stress Response
Posted 17th August 2018 by Kate Barlow
In an article which discussed the exploration of the ‘orphan crop’ pearl millet, Amibka Dudhate concluded that photosynthesis, plant hormone signal transduction, and mitogen-activated protein kinase signalling pathways are significantly activated on the advent of drought stress in pearl millet. Her colleague, Harshraj Shinde, expands on these findings.
The Long Shadow of the European Ruling on New Breeding Technologies
Posted 1st August 2018 by Kate Barlow
Last week, the European Court of Justice ruled that gene-edited crops are equivalent to transgenic GMOs. The court ruling came as a surprise because it negates a preliminary opinion that was issued by the court’s Advocate General Michael Bobek in January 2018. This reactionary ECJ ruling might become the final nail in the coffin of the European Agbiotech sector and many scientists, including myself, are concerned that it will discourage the use of genome editing in agriculture.
Novel Photosynthesis Technology – the Solution to the Global Food Security Crisis?
Posted 23rd July 2018 by Kate Barlow
Ensuring global food security
The human population is increasing, which means that we need to improve crop productivity to maintain food security. Over the last century, plant breeding and modern agriculture have made large gains in productivity. However, this growth is not keeping pace with demand. If plant photosynthesis could be improved, this would provide breeders with a new tool to increase crop yields. This has been a major research focus over the last 20 years and significant progress has been made in understanding this process. However, crops with improved photosynthesis have yet to be successfully commercialised.
