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PCR

Nucleic Acid Quantification, Mutation Enrichment Technologies & Streamlining Genotyping

In just two years’ time, the digital PCR and qPCR market is set to be worth an estimated $4.94 billion. Dedicated sessions at the 4BIO Summit covered topics such as gene expression analysis, liquid biopsies and high-throughput screening. We have made the following presentation slides from Hendrik Emons, Naomi Park & Mike Makrigiorgos available.

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Speaker profile: Jari Louhelainen

With many years experience in both cancer research and forensics, we spoke to Associate Professor of Biochemistry Jari Louhelainen about his career so far and what he thinks the future holds for molecular biology.

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DNA is no longer just ACGT: synthetic DNA is here to stay

One of the most amazing aspects surrounding us is life itself – not just humans, but the environment: trees, flowers, insects, animals and even bacteria. They all share one central molecule which is crucial for their existence.

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Digital-Droplet PCR Gets Another ‘D’

Triple-d PCR enhances digital PCR sensitivity and precision for liquid biopsies

Digital-droplet PCR (ddPCR) has been implemented in diverse fields such as cancer biomarkers, viral load detection, prenatal screening, organ donor rejection, or library assessment for next generation sequencing. Detection of emerging resistance or minimal residual disease via ddPCR in liquid biopsies is also growing rapidly.

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Case Study: High-throughput SNP Screening Downstream of CRISPR/Cas9 Gene Editing

One of the most powerful applications of genome editing is the introduction of nucleotide substitutions in specific genomic sites. This can be used to mimic single-nucleotide polymorphisms (SNPs) or to generate stop codons that yield precise gene knockouts. However, screening hundreds of clones for a single edited nucleotide remains a challenge, especially in the absence of a corresponding phenotype.

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Busting Common Myths about Quantitative PCR & Digital PCR

qPCR and dPCR are mature analytical methods for quantifying selected genetic materials in a sample. Both utilise the PCR method to amplify amounts as small as a single molecular fragment (template) to levels where they can be easily detected, usually by fluorescence techniques. 

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The Big List of Quantitative PCR & Digital PCR Companies & Investors

The global qPCR & dPCR instrumentation market is growing and is expected to reach 1631.09 million USD by the end of 2025[1].

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Connecting Cancer Biology to Clinical Oncology: Still a World to Win

Our understanding of the aberrant biological pathways (oncogenic pathways) that are involved in the formation and progression of cancers has increased with huge leaps in the last decades of cancer research. The ever-increasing knowledge was and still is accompanied by the development of increasing numbers of precision drugs, tailored to neutralize these aberrations.

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