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PCR

How To Avoid Unnecessary Data Loss In qPCR Reactions

Analysing qPCR data has always been based on setting a quantification threshold and determining the Cvalue which is the fractional number of cycles needed to reach the threshold.

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Molecular Beacons In Multiplex Digital PCR Assays

Every droplet of a digital PCR assay can contain as many as 35 different highly specific molecular beacon probes that are each tagged with a different combination of three out of seven available fluorescent colours.

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Pre-PCR: Reducing Biases Without Losing DNA Material

Peter Rådström is an expert in the area of food microbiology at the University of Lund, Sweden. He particularly focuses on carbohydrate metabolism in lactic acid bacteria and neurotoxin formation in Clostridium botulinum and Staphylococcus aereus.

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Pre-PCR: When Generating PCR-Compatible Samples Becomes Possible

Peter Rådström’s research is about the developments of pre-PCR processing, a method which aims to generate PCR-compatible samples. Diagnostic PCR is often limited by components that interfere with the amplification of PCR inhibitors and pre-PCR can overcome PCR inhibition without impairing the detection limit, addressing all steps leading to PCR amplification, samples treatment and PCR chemistry. This lowers the need for pure extracts, leading to simplified analysis processes and improved detection limits.

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PCR Applications In Host Residual DNA Samples

Host residual DNA (hrDNA) as an impurity in biologic drugs is currently quantified by DNA extraction followed by qPCR. Musaddeq Hussain developed a method for direct qPCR without using DNA extraction from mAb drugs. He gave us his view about the research and challenges of working in the field.

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Aneuploid Screening Of Embryonic Stem Cell Clones By Digital PCR

Researchers at the Institute Clinique de la Souris investigate the role of genetics in health and disease concentrating on experimental variation of genome sequence in mouse models.

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Challenges And Applications In Using qPCR and Digital PCR For Environmental Samples

The detection of the origin of faecal pollution in complex watersheds is beginning to take a prominent place in hazard identification and risk management policies. 

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