Posted 26th July 2017 by Fabio Caligaris
Analysing qPCR data has always been based on setting a quantification threshold and determining the Cq value which is the fractional number of cycles needed to reach the threshold.
Posted 21st June 2017 by Fabio Caligaris
Every droplet of a digital PCR assay can contain as many as 35 different highly specific molecular beacon probes that are each tagged with a different combination of three out of seven available fluorescent colours.
Posted 5th June 2017 by Fabio Caligaris
1. Analysis Of Different Approaches For The Selection Of Reference Genes In RT-qPCR Experiments: A Case Study In Skeletal Muscle Of Growing Mice, International Journal of Molecular Science
Posted 24th May 2017 by Fabio Caligaris
Peter Rådström is an expert in the area of food microbiology at the University of Lund, Sweden. He particularly focuses on carbohydrate metabolism in lactic acid bacteria and neurotoxin formation in Clostridium botulinum and Staphylococcus aereus.
Posted 21st April 2017 by Fabio Caligaris
Peter Rådström’s research is about the developments of pre-PCR processing, a method which aims to generate PCR-compatible samples. Diagnostic PCR is often limited by components that interfere with the amplification of PCR inhibitors and pre-PCR can overcome PCR inhibition without impairing the detection limit, addressing all steps leading to PCR amplification, samples treatment and PCR chemistry. This lowers the need for pure extracts, leading to simplified analysis processes and improved detection limits.
Posted 14th April 2017 by Fabio Caligaris
Host residual DNA (hrDNA) as an impurity in biologic drugs is currently quantified by DNA extraction followed by qPCR. Musaddeq Hussain developed a method for direct qPCR without using DNA extraction from mAb drugs. He gave us his view about the research and challenges of working in the field.
Posted 3rd April 2017 by Fabio Caligaris
Researchers at the Institute Clinique de la Souris investigate the role of genetics in health and disease concentrating on experimental variation of genome sequence in mouse models.
Posted 27th March 2017 by Fabio Caligaris
The detection of the origin of faecal pollution in complex watersheds is beginning to take a prominent place in hazard identification and risk management policies.