In its first part, this workshop will provide an introduction to digital PCR including its principle, related statistics, methods for sample partition and commercially available digital PCR systems.
The second part of the workshop will treat technological innovations comprising the microfluidic integration of droplet generation, amplification and detection into one single cavity, as well as probe techniques for increased signal-to-noise ratio, improved capabilities for multiplexing and SNP detection. Futhermore, lysis strategies required for single cell digital PCR will be reviewed, with special focus on process alternatives for lysis of bacteria.
Thirdly, in a live lab we will demonstrate how digital nucleic acid amplification can be performed using standard laboratory devices only (i.e mini-centrifuge, incubator, fluorescence reader), enabled by a novel microfluidic chip. In addition, we present a laboratory prototype for sample-to-answer digital nucleic amplification.
Felix von Stetten, Associate Director of Hahn-Schickard, Freiburg, Germany
THE WORKSHOP WILL BE HELD AT THE SINGH CENTER.
JOIN US FOR A DRINKS RECEPTION AND TOUR OF THIS OUTSTANDING FACILITY AT 6 PM.
Located in Philadelphia, PA, the Singh Center for Nanotechnology is a nanotechnology and microfluidics research center that opens its doors to all of academia and industry. The Singh Center currently houses over 90+ pieces of instrumentation for the fabrication, characterization and measurement of nanomaterials and devices in a new state of the art building specifically designed for nanotechnology research.
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