Workshop: Innovations in digital nucleic acid amplification

When

24th Jul 2017
9am-1pm

Where

Philadelphia
Singh Center

Innovations in digital nucleic acid amplification Workshop

In its first part, this workshop will provide an introduction to digital PCR including its principle, related statistics, methods for sample partition and commercially available digital PCR systems.

The second part of the workshop will treat technological innovations comprising the microfluidic integration of droplet generation, amplification and detection into one single cavity, as well as probe techniques for increased signal-to-noise ratio, improved capabilities for multiplexing and SNP detection. Futhermore,  lysis strategies required for single cell digital PCR will be reviewed, with special focus on process alternatives for lysis of bacteria.

Thirdly, in a live lab we will demonstrate how digital nucleic acid amplification can be performed using standard laboratory devices only (i.e mini-centrifuge, incubator, fluorescence reader), enabled by a novel microfluidic chip. In addition, we present a laboratory prototype for sample-to-answer digital nucleic amplification.

Synopsis

  • Introduction
    • Digital PCR and related statistics
    • Overview on commercial systems for digital nucleic acid amplification
  • Innovations
    • Digital nucleic amplifications using standard laboratory devices
    • Microfludic integration of droplet generation, amplification and detection into one single device
    • Mediator Probe PCR for improved signal to-noise-ratio, multiplexing and SNP detection
    • Protocols for amplification-compatible lysis of single cells and bacteria in droplets
  • Life lab / Demonstrators
    • Microfluidic chip for droplet generation, digital amplification and detection based on standard laboratory devices
    • Microfluidic integration of a complete workflow into one single cartridge: From nasal swab sample to digital amplification

Workshop Leader

Felix von Stetten, Associate Director of Hahn-Schickard, Freiburg, Germany

Felix Von Stetten75                    Hahn-Schickard300

Venue

Join us at the Singh Center

SINGH LOGO_color300x250

THE WORKSHOP WILL BE HELD AT THE SINGH CENTER. 

JOIN US FOR A DRINKS RECEPTION AND TOUR OF THIS OUTSTANDING FACILITY AT 6 PM.

Sing Combined 900x500Located in Philadelphia, PA, the Singh Center for Nanotechnology is a nanotechnology and microfluidics research center that opens its doors to all of academia and industry. The Singh Center currently houses over 90+ pieces of instrumentation for the fabrication, characterization and measurement of nanomaterials and devices in a new state of the art building specifically designed for nanotechnology research.

Discover more about the Singh Center

Register

Step 1: Click to Choose Your Delegate Package

Standard Fee

$200.00

Click to select

Academic/Hospital/NFP Fee

$200.00

Click to select

Solution Provider Fee

$200.00

This fee applies to organisations offering services and/or business & technology solutions and also to business consultants.

We accept the following cards through Worldpay
  • Visa
  • Visa Electron
  • Mastercard
  • Maestro
  • JCB

Step 2: Delegate Information