Workshop: Tataa Biocenter

When

27th-28th Jul 2017

Where

Philadelphia
Venue to be confirmed

How to get good quality control in qPCR

Who Should Attend?

This course will go deep into the MIQE guidelines, describe the important steps in RNA and DNA analysis with qPCR and how you should work to fulfil the guidelines. To follow the guidelines will give you a better control of the quality of your results. The course is theoretical with a few practical computer-based exercises.

Workshop Leader

Mikael Kubista, CEO, Tataa Biocenter & Head of Department, BTU, CAS

Mikael Kubista (3)                       

Dr Kubista is the head of the department of gene expression at the institute of Biotechnology of the Czech Academy of Sciences, and founder and CEO of the TATAA Biocenter (www.tataa.com). He was one of the pioneers contributing to the development of quantitative real-time PCR (qPCR) and introduced qPCR for single cell expression profiling. He led the development of reagents for high throughput single cell expression profiling and quality control at TATAA. He also developed qPCR tomography for intracellular expression profiling. Kubista co-authored the Minimum Information for Publication of Quantitative. Real-Time PCR Experiments (MIQE) guidelines, was partner of the SPIDIA project, and is member of the CEN and ISO workgroups drafting the forthcoming guidelines on pre-analytics.

Synopsis

 

Background about the MIQE guidelines

  • What are the MIQE guidelines?
  • Why are they important?

What to think about when doing experimental design

How to do nucleic acid extraction and quality control of extracts

  • Different approaches for nucleic acid extraction
  • How can we check the quality of extracted samples?
  • How do we identify inhibitory samples?

The Reverse Transcription reaction

  • Different priming strategies for reverse transcription
  • Pros and cons for different strategies

How to do primer and probe design

  • How to search for gene sequences
  • Which are the factors that affect design?
  • How do we avoid primer dimer formation?
  • Other important considerations for primer design
  • How to design hydrolysis probes
  • Practical exercises in primer design

How to optimize qPCR assays

  • Which factors affect the PCR?
  • Which factors can be optimized?

How to validate qPCR assays, LOD and LOQ

  • How to determine LOD and LOQ
  • Precision estimation
  • Which controls to use

Data analysis

  • How does qPCR software process the data?
  • How to evaluate curves and set threshold

Normalization

  • Different ways to normalize
  • How to find stable reference genes

How to do relative quantification

  • Quantification methods and equations
  • How to interplate calibration

Absolute quantification strategies

  • What is a suitable standard?
  • How to do absolute quantification

Dr Kubista is the head of the department of gene expression at the institute of Biotechnology of the Czech Academy of Sciences, and founder and CEO of the TATAA Biocenter (www.tataa.com). He was one of the pioneers contributing to the development of quantitative real-time PCR (qPCR) and introduced qPCR for single cell expression profiling. He led the development of reagents for high throughput single cell expression profiling and quality control at TATAA. He also developed qPCR tomography for intracellular expression profiling. Kubista co-authored the MIQE guidelines, was partner of the SPIDIA project, and is member of the CEN and ISO workgroups drafting the forthcoming guidelines on pre-analytics.

Other Workshops

Microfluidics and Lab on a chip technologies for commercial product development: strategies, technologies, markets and applications.

NGS Clinical Applications workshop: Basic Principles of NGS Assay Design and Validation; Basic principles of informatics: Precision Medicine using NGS.

Venue

Join us in Philadelphia

THE WORKSHOP WILL BE HELD AT

Wyndham Philadelphia Historic District
400 Arch Street
Philadelphia, PA 19106
USA

Find our more.

 

 

 

Register

Step 1: Click to Choose Your Delegate Package

Standard Fee

$699.00

Click to select

Academic/NFP Fee

$499.00

Click to select

Solution Provider Fee

$699.00

This fee applies to organisations offering services and/or business & technology solutions and also to business consultants.

We accept the following cards through Worldpay
  • Visa
  • Visa Electron
  • Mastercard
  • Maestro
  • JCB

Step 2: Delegate Information