Digital PCR: Possibilities & Opportunities
Posted 23rd April 2018 by Jane Williams
The Digital PCR track at the 4Bio Summit covered the benefits and future development of dPCR, comparisons between dPCR and qPCR and other technologies such as NGS, strategies to convert to a dPCR platform and applying the technology to precision medicine.
If you weren’t able to attend the 4Bio Summit, enjoy these free slides from Ward De Speigelaere, Anthony Magliocco and Nasrin Sarafan-Vasseur.
Prospects for Digital PCR in Absolute Quantification of DNA & RNA
Using examples of virology, Ward De Speigelaere looks at the possibilities and pitfalls of current digital PCR technology for absolute quantification of DNA and RNA. These slides will explore the methods for normalization of RNA data in digital PCR.
Challenges and Opportunities for Digital PCR in the CLIA of the Moffitt Cancer Experience
Circulating Tumor DNA Detection from Heparinized Plasma Samples by Droplet Digital PCR
Heparin is often used as plasma anticoagulant for tumor marker analysis but corresponds also to an inhibitory of PCR not enabling circulating tumor DNA (ctDNA) detection, which has been highlighted as a potential “liquid biopsy”.
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