Pre-PCR: Reducing Biases Without Losing DNA Material
Posted 24th May 2017 by Jane Williams
Peter Rådström is an expert in the area of food microbiology at the University of Lund, Sweden. He particularly focuses on carbohydrate metabolism in lactic acid bacteria and neurotoxin formation in Clostridium botulinum and Staphylococcus aereus.
In his current investigation he applies pre-PCR procedures to overcome PCR inhibitors and avoid biases in PCR results. The concept behind pre-PCR reaction is to bypass the impact of potential inhibitors and reduce the number of worksteps within the PCR workflow. Nevertheless, the identity of PCR inhibitors is often unknown and the traditional workflow is quite laborious when it comes to handling them.
Pre-PCR processing encompasses a series of steps occurring before the detection of PCR products. These are sampling, sample preparation and DNA amplification. The aim of this methodology is to adapt a complex biological model and its target nucleic acids into PCR-amplifiable samples via the preparation of specimens and amplification conditions (Rådström et al., 2004).
Pre-PCR applications allow a lower use of pure extracts, thus leading to simplified analysis processes and improved detection limit. So far, it has been applied to improve molecular diagnostics, forensics as well as next generation sequencing.
Watch the full video of his presentation.
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