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Tag: qPCR

CE and HPLC applications for rAAV characterisation

Despite recent advances in the gene therapy field, significant challenges lie ahead for the consistent manufacturing and release of these medicines. Analytical methods and automation need to keep pace as more medicines move towards the market and more quality control resources are required. Well-developed methods on robust platforms will be crucial to ensure the quality of these novel medicines for the doctors and patients who need them.

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Gut Microbiome analysis – Getting it right

The cascade of new discoveries relating health and disease to our gut microbiome has spurred the notion that we now find ourselves in the middle of a “microbiome revolution”. Just to mention some recent examples, mechanisms have been demonstrated for gut bacteria contributing to Parkinson’s disease,[1] determining response to immune checkpoint inhibitor cancer therapy,[2] and even autistic behavior when fecal material from autistic children was transplanted into mice.[3]

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Nucleic Acid Quantification, Mutation Enrichment Technologies & Streamlining Genotyping

In just two years’ time, the digital PCR and qPCR market is set to be worth an estimated $4.94 billion. Dedicated sessions at the 4BIO Summit covered topics such as gene expression analysis, liquid biopsies and high-throughput screening. We have made the following presentation slides from Hendrik Emons, Naomi Park & Mike Makrigiorgos available.

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Rabies diagnostics with RT-PCR assays, Liquid Diagnostics and What the Future Holds for Digital PCR

With its reduction in cost, and an increased number of real-time PCR users purchasing Digital PCR, the qPCR and Digital PCR market is predicted to grow to $4.94 billion by 2021. At the 4BIO event in San Francisco, three dedicated tracks proved a timely opportunity to learn first-hand about dPCR whilst keeping up-to-date with latest market developments and strategies. If you weren’t able to be there in person, these slides are now available from Hestia Mellert, Olivier Thas and Yu Li.

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Busting Common Myths about Quantitative PCR & Digital PCR

qPCR and dPCR are mature analytical methods for quantifying selected genetic materials in a sample. Both utilise the PCR method to amplify amounts as small as a single molecular fragment (template) to levels where they can be easily detected, usually by fluorescence techniques. 

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The Big List of Quantitative PCR & Digital PCR Companies & Investors

The global qPCR & dPCR instrumentation market is growing and is expected to reach 1631.09 million USD by the end of 2025[1].

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Digital PCR: Possibilities & Opportunities

The Digital PCR track at the 4Bio Summit covered the benefits and future development of dPCR, comparisons between dPCR and qPCR and other technologies such as NGS, strategies to convert to a dPCR platform and applying the technology to precision medicine.

If you weren’t able to attend the 4Bio Summit, enjoy these free slides from Ward De Speigelaere, Anthony Magliocco and Nasrin Sarafan-Vasseur.

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The Development and Commercialisation of Robust Circulating DNA & RNA Tests

Precision medicine in oncology requires matching each patient with treatment personalised to their genes and often the proteomic profile of their cancer. Liquid biopsies continue to gain grounds as a tool for diagnostic testing in oncology.

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