A Focus On Genome Editing And Bioinformatics
Posted 10th May 2017 by Jane Williams
During the 5th Plant Genomics and Gene Editing Congress, experts in the field of bioinformatics and genome sequencing shared their contributions in plant research. We made some of their presentation slides available for you to download.
A Unified Expression Platform That Makes Tomato Suitable Model For Exploring Plant-Environment Interactions
RNA-Seq has become in recent years a powerful tool for transcriptome investigations. Nevertheless, computational methods applied for the analysis of large data sequencing still need to be standardised [1]. TomExpress is a unified tomato RNA-Seq platform gathering raw sequence data available in public database, thus avoiding major biases resulting from the use of different mapping and statistical processing profiles. Professor Zouine’s presentation conveys a detailed explanation of its uses and applications. View presentation slides here.
Targeted Breeding Applications Of CRISPR-Cas Technology for European Markets
CRISPR-Cas technology is one of the latest advances in plant breeding. This technology is used to perform several methods, such as targeted single and multiple gene knock-outs, introduction of single-nucleotide polymorphism into a gene of interest and fluorescent proteins expression. The CRISPR-Cas method makes possible a better exploitation of crop genetic diversity, thus developing novel and more efficient seed products. In this presentation, Professor Gutterson covers the potential product targets of CRISPR including the latest improvements in wheat genome. View presentation slides here.
Toward A Better Understanding of Plant Genome Structure: Combining NGS, Optical Mapping Technology And CRISPR-CATCH Approach
Agriculture is currently facing multiple challenges due to climate change and limited energy resources, hence making a thorough understanding of plant evolution and adaptation a major goal in plant research. Nevertheless, the NGS technologies are still dealing with problems in sequencing plant genomes particularly due to genomes complexity and sizes. Professor Berges’ research focuses on improving a whole genome assembly using hybrid scaffolding combining PacBio sequencing and Bionano Genomics mapping data. Her team is also developing a new sequence capture approach for large DNA fragments using the CATCH method. View presentation slides here.
Odyssey Of The IWGSC Reference Genome Sequence: 12 Years 1 Month 28 Days 11 Hours 10 Minutes And 14 Seconds
Wheat presents a large and hexaploid genome. The IWGSC is an international and collaborative consortium aiming to produce a high-quality reference of this genome, accelerating wheat genetics improvements and produce physical maps for 21 individual bread wheat chromosomes. In this presentation Professor Eversole shows the achievements reached so far and the future goals set by IWGCS to release annotated sequences of other varieties that present the breath of the wheat gene pool. View presentation slides here.
Click here to access more presentation slides about the novelties in genome engineering and plant microbiome.
Reference:
- Maza, E., Frasse, P., Senin, P., Bouzayen, M. and Zouine, M. (2013). Comparison of normalization methods for differential gene expression analysis in RNA-Seq experiments: A matter of relative size of studied transcriptomes. Communicative & Integrative Biology 6 (6), e25849.
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