Multiplex Super-Selective PCR Assays for the Detection and Quantitation of Rare Somatic Mutations
Posted 22nd February 2021 by Nicholas Noakes
Professor Fred Kramer spoke at the recent Research & Technology Series exploring Flow Cytometry / qPCR & Digital PCR / Liquid Biopsies. During his presentation, he explained how Super Selective primers enable the simultaneous identification and quantitation of rare somatic mutations in routine multiplex PCR assays, while virtually eliminating signals from abundant closely related wild-type sequences.
Nucleic Acid Quantification, Mutation Enrichment Technologies & Streamlining Genotyping
Posted 30th January 2019 by Jane Williams
In just two years’ time, the digital PCR and qPCR market is set to be worth an estimated $4.94 billion. Dedicated sessions at the 4BIO Summit covered topics such as gene expression analysis, liquid biopsies and high-throughput screening. We have made the following presentation slides from Hendrik Emons, Naomi Park & Mike Makrigiorgos available.
Speaker profile: Jari Louhelainen
Posted 16th January 2019 by Jane Williams
With many years experience in both cancer research and forensics, we spoke to Associate Professor of Biochemistry Jari Louhelainen about his career so far and what he thinks the future holds for molecular biology.
DNA is no longer just ACGT: synthetic DNA is here to stay
Posted 23rd November 2018 by Jane Williams
One of the most amazing aspects surrounding us is life itself – not just humans, but the environment: trees, flowers, insects, animals and even bacteria. They all share one central molecule which is crucial for their existence.
Digital-Droplet PCR Gets Another ‘D’
Posted 2nd November 2018 by Jane Williams
Triple-d PCR enhances digital PCR sensitivity and precision for liquid biopsies
Digital-droplet PCR (ddPCR) has been implemented in diverse fields such as cancer biomarkers, viral load detection, prenatal screening, organ donor rejection, or library assessment for next generation sequencing. Detection of emerging resistance or minimal residual disease via ddPCR in liquid biopsies is also growing rapidly.
Case Study: High-throughput SNP Screening Downstream of CRISPR/Cas9 Gene Editing
Posted 31st October 2018 by Jane Williams
One of the most powerful applications of genome editing is the introduction of nucleotide substitutions in specific genomic sites. This can be used to mimic single-nucleotide polymorphisms (SNPs) or to generate stop codons that yield precise gene knockouts. However, screening hundreds of clones for a single edited nucleotide remains a challenge, especially in the absence of a corresponding phenotype.
Busting Common Myths about Quantitative PCR & Digital PCR
Posted 4th June 2018 by Jane Williams
qPCR and dPCR are mature analytical methods for quantifying selected genetic materials in a sample. Both utilise the PCR method to amplify amounts as small as a single molecular fragment (template) to levels where they can be easily detected, usually by fluorescence techniques.
The Big List of Quantitative PCR & Digital PCR Companies & Investors
Posted 30th May 2018 by Jane Williams
The global qPCR & dPCR instrumentation market is growing and is expected to reach 1631.09 million USD by the end of 2025.