5 PCR Articles We’d Recommend This Month
Posted 5th June 2017 by Jane Williams
1. Analysis Of Different Approaches For The Selection Of Reference Genes In RT-qPCR Experiments: A Case Study In Skeletal Muscle Of Growing Mice, International Journal of Molecular Science
Pre-PCR: Reducing Biases Without Losing DNA Material
Posted 24th May 2017 by Jane Williams
Peter Rådström is an expert in the area of food microbiology at the University of Lund, Sweden. He particularly focuses on carbohydrate metabolism in lactic acid bacteria and neurotoxin formation in Clostridium botulinum and Staphylococcus aereus.
Pre-PCR: When Generating PCR-Compatible Samples Becomes Possible
Posted 21st April 2017 by Jane Williams
Peter Rådström’s research is about the developments of pre-PCR processing, a method which aims to generate PCR-compatible samples. Diagnostic PCR is often limited by components that interfere with the amplification of PCR inhibitors and pre-PCR can overcome PCR inhibition without impairing the detection limit, addressing all steps leading to PCR amplification, samples treatment and PCR chemistry. This lowers the need for pure extracts, leading to simplified analysis processes and improved detection limits.
PCR Applications In Host Residual DNA Samples
Posted 14th April 2017 by Jane Williams
Host residual DNA (hrDNA) as an impurity in biologic drugs is currently quantified by DNA extraction followed by qPCR. Musaddeq Hussain developed a method for direct qPCR without using DNA extraction from mAb drugs. He gave us his view about the research and challenges of working in the field.
Aneuploid Screening Of Embryonic Stem Cell Clones By Digital PCR
Posted 3rd April 2017 by Jane Williams
Researchers at the Institute Clinique de la Souris investigate the role of genetics in health and disease concentrating on experimental variation of genome sequence in mouse models.
Challenges And Applications In Using qPCR and Digital PCR For Environmental Samples
Posted 27th March 2017 by Jane Williams
The detection of the origin of faecal pollution in complex watersheds is beginning to take a prominent place in hazard identification and risk management policies.
The Potential Advantages of Digital PCR When Performing Molecular Measurement
Posted 20th February 2017 by Jane Williams
Jim Huggett’s research focuses on molecular diagnostics, genomics and nucleic acids, including cancer and foetal genetic analysis and the diagnosis of infectious diseases. We interviewed him about the latest developments and the latest advances in qPCR research.
New Developments in Liquid Biopsy Technology
Posted 13th January 2017 by Jane Williams
Advances in liquid biopsy technology have seen the development of novel methods that allow for different applications in genetic disorders and tumour detection.
