Training Course: Machine Learning Analysis on Single Cell Data

When

9^th-10^th Jul 2024

Where

Singapore

Register Online

Send me full training programme when it is ready

The purpose of this workshop is to tear back the complexity behind single cell analysis. Participants will learn practical skills for analyzing single cell datasets and develop a conceptual understanding of the machine learning foundations behind each method. Participants will also receive an introduction to emerging trends in single cell analysis such as deep learning.

All attendees of this training program will have full access to the Single Cell Congress: Asia taking place the next day

Course Trainers

List of trainers will be updated soon…

Course Programme

DAY 1

DATA IMPORT AND QC

File format and read-in methods (sparseMatrix vs. Matrix benefits)
Download from public repo and read into as Seurat object
Gross QC (special note on ambient RNA for single-nuclei data)
Multiplet removal (using scDblFinder)

DATA PROCESSING AND VISUALISATION

Standard pre-processing (Data normalization, hvg, scaling, PCA, UMAP)
Visualization (FeaturePlot, plot_density) & databases for markers
Clustering (choosing number of PC, resolution, DimPlot)

DAY 2

CELL TYPE ANNOTATION

Differential expression between clusters (FindAllMarkers)
Cell type annotation (DISCO, RCAv2?)
Cell type specific QC

WORKING WITH MULTIPLE SAMPLES

Read in multiple samples and augment the metadata
Differential expression (FindMarkers vs. DESeq2)
Checking for batch effect and data integration (Seurat, harmony)
Overview of downstream tools (Cell Chat, GRN, trajectory) and conclusion

Training venue

The exact training venue in Singapore will be announced soon… stay tune.

Send me full training programme when it is ready

PRE-WORKSHOP
This pre-workshop commences virtually 2 weeks before the training. People who are already familiar with R can skip this part

Installation – PDF document & group clinic hour
Introduction to R – youtube playlist.
Welcome session (2 hrs in-person) – clear doubts
Homework – submit via email. Later send model solution
Intro to single-cell experiment and study design – Nearpod / video

For registration and further inquiry kindly contact:

[email protected] (UK office)
[email protected] (Asia office)

Register

Step 1: Click to Choose Your Delegate Package

Training pass (1 attendee)*

$999.00

Price is in USD for 1 pax for 2-day training course. *Subject to final review

We accept the following cards through Worldpay