Virtual Workshop: 10X Genomics

Understanding diversity and heterogeneity in the context of disease

10x Genomics

Uncover molecular insights, dissect cell-type differences, investigate the adaptive immune system, detect novel subtypes and biomarkers, and map the epigenetic landscape cell by cell. Enabling deeper insight into cancer, immunology, neuroscience, and developmental biology, 10x Genomics gives researchers the ability to see biology at true resolution.

https://www.10xgenomics.com

Agenda

14:00 – 14:05 Welcome and Introductions

14:05 – 14:25 Understanding diversity and heterogeneity in the context of disease
Assessing gene expression with morphological context is critical to our understanding of biology and the progression of disease. Historically, it has been challenging to spatially interrogate complex heterogeneous tissues in a high-throughput manner, especially without previously generated assumptions about the genes being expressed. With Visium Spatial Solutions researchers can now map the whole transcriptome with morphological context.
Join us for this seminar to learn how Visium Spatial Solutions from 10x Genomics give you a comprehensive understanding of the relationships between cellular function, phenotype, relationships, and location in intact tissue sections. Enabling deeper insight into cancer, immunology, neuroscience, developmental biology, and beyond, Visium Spatial Solutions give researchers the ability to discover more by seeing biology at true resolution. Discover novel insights into normal development, disease pathology, and clinical translational research.

Christophe Fleury, Senior Science & Technology Advisor, 10x Genomics

14:25 – 14: 45 Genome-wide Spatial Expression Profiling in FFPE tissues
Formalin-fixed paraffin embedding (FFPE) is the most widespread long-term tissue preservation approach. We developed a procedure to perform genome-wide spatial analysis of mRNA in FFPE tissue sections. The procedure takes advantage of well-established, commercially available methods for imaging and spatial barcoding using slides spotted with barcoded oligo(dT) probes to capture the 3’ end of mRNA molecules in tissue sections. First, we conducted expression profiling and cell type mapping in coronal sections from the mouse brain to demonstrate the method’s capability to delineate anatomical regions from a molecular perspective. Second, we explored the spatial composition of transcriptomic signatures in ovarian carcinosarcoma samples using data driven analysis methods, exemplifying the method’s potential to elucidate molecular mechanisms in heterogeneous clinical samples.
Eva Gracia Villacampa, Dept. of Gene Technology, KTH, Science for Life Laboratory

14:45 – 14:55 Q&A