Post-conference workshop: NGS library construction and quality control

When

29^th Nov 2018
8.30am-4.30pm

Where

Rotterdam, Netherlands
Postillion Convention Centre WTC Rotterdam

Post-conference workshop: NGS library construction and quality control

NGS library construction and quality control – finding the right approach, kit and provider in the jungle

Hosted by Filip Stern

NGS project manager
Filip has worked in the field of NGS for several years, both within the academic and the private sector. Currently he is responsible for NGS activities at TATAA Biocenter and has been both developing and validated NGS methods/library preparations. He has experience from a broad range of different NGS applications throughout various collaborations

Description:

This course gives an introduction to Next Generation Sequencing (NGS) and its many applications. The course will focus on considerations for the NGS experiment design, the different sequencing platforms, quality control of samples, library preparation techniques, and quantification of libraries for sequencing.

Introduction to NGS:

The history of DNA sequencing
What are the advantages with NGS? Disadvantages?
What is coverage, index, multiplexing, depth etc…

Applications:

DNA – Whole Genome Sequencing (WGS), targeted resequencing etc.
RNASeq – Expression analysis, Splice variants detection etc.
Metagenomic sequencing
Epigenetic sequencing
miRNA

Sample preparation and quality control:

Which starting material can be used for NGS?
Which quality controls are included in the experimental workflow?
How to choose the right library prep protocol.

Instrumentation:

Which platforms are available?
How do they work?
Which platform is suitable for my experiment?

Venue

JOIN US AT THE POSTILLION CONVENTION CENTRE WTC ROTTERDAM

THE WORKSHOP WILL BE HELD AT THE POSTILLION CONVENTION CENTRE WTC ROTTERDAM

• A non-stop high-speed train runs between Rotterdam and Schiphol (Amsterdam airport) every 20 minutes. The journey time is 20 minutes. Cost €22 Euros
• Located in the city centre (surrounded by shops, architecture, museums, restaurants and the Port of Rotterdam)
• Walking distance from the international train station
• Rotterdam – The Hague airport with flights to at least 30 international destinations is a short taxi ride
• A large number of parking facilities can be found in the area
• A wide variety of hotels are within walking distance. (Details will be sent to you in your welcome letter when you register)

In the vibrant heart of the Rotterdam metropolis: you cannot get more central than this. Wherever you are in the world, a World Trade Center is a special place. It is an (inter)national corporate community, in a unique building that meets the highest standards. From high-spec office space to high-tech meeting rooms, shops, restaurants and a wide range of additional services.