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PCR, Flow Cytometry & Life Science Technology Resources

Welcome to the PCR, Flow Cytometry and Life Science Technology resources page: a curated list of presentation videos, slide decks, reports and posters.

These resources were produced from our PCR Flow Cytometry, Microfluidic, Liquid Biopsies and other research & technology series of events.

Upcoming Meetings

Presentation Videos

Circulating biomarkers in melanoma immunotherapy

Genevieve Boland, Director, Surgical Oncology Research Laboratories , Harvard University

Extracellular Vesicles: from cell-cell communication to biomarkers discovery

Bruno Costa-Silva, Systems Oncology, Group Leader, Champalimaud Foundation

Clinical-oriented applications for Imaging Flow Cytometry

Ziv Porat, Head of Flow Cytometry Unit, Weizmann Institute of Science, Israel

Flow based MRD assessment for clinical decision making in acute myeloid leukaemia

Jacqueline Cloos, Professor, Amsterdam UMC

Robust, reproducible & interactive QC, cell population identification and biomarker discovery

Ryan Brinkman, Professor, Distinguished Scientist, British Columbia Cancer Research Center, Canada

Recent developments of the extracellular vesicle measurements by flow cytometry

Romaric Lacroix, Professor in Hematology, Aix-Marseille University, France

Isothermal Digital and Multiplex Detection of microRNA biomarkers

Guillaume Gines, Postdoctoral researcher, Gulliver Laboratory – ESPCI Paris / PSL Research University

Beckman Coulter: Introducing Cytoflex SRT

Derek Davies, Francis Crick Institute; Dr Peter O’Toole, University of York; Karen Hogg, University of York.

Automated Threshold Determination Methods in dPCR – Getting from Zero to One

Wim Trypsteen, Postdoctoral Researcher & Assisting Academic Staff, Faculty of Medicine and Health Sciences, Ghent University, Belgium

Multiplex Super Selective PCR Assays for the Detection and Quantitation of Rare Somatic Mutations Title

Fred Kramer, Professor of Microbiology, Biochemistry and Molecular Genetics, Rutgers University, USA

Challenges and Opportunities for Digital PCR in the CLIA Laboratory of the Moffit Cancer Experience

Anthony Magliocco, Senior Member, Moffit Cancer Center

A Digital High Resolution Melt Platform Enables Single Molecule Sensitive, Large-Scale Sequence Profiling

Stephanie Fraley, Assistant Professor, Bioengineering, UCSD

Quality Control and Technical Variance in Plant qPCR Analysis

Jose Die, Visiting Scientist, United States Department of Agriculture

Tumor Derived DNA in Cerebral Spinal Fluid for the Management of Brain Tumours

Chetan Bettegowda, Associate Professor of Neurosurgery, John Hopkins Medicine

Selfie-dPCR: Absolute measurement of gene expression and replication

Petar Podlesniy, Scientific Researcher, CiberNed, IIBB, Barcelona, Spain

Two-tailed PCR for Precision Diagnostics

Mikael Kubista, Professor, Czech Academy of Sciences; Chairman of the Board, Founder, TATAA Biocenter, Sweden

MSRE-qPCR for multiplexed analysis of DNA methylation can be accurately used for detection and validation of colorectal cancer-specific biomarkers in liquid biopsy samples

Kristi Kruusmaa, Head of Research, Universal DX Diagnostics, Spain

Identification of rare mutations and DNA methylation patterns in cell-free DNA using multiplexed Enhanced-ice-COLD-PCR

Jörg Tost, Director, Laboratory Epigenetics and Environment, National Center for Research in Human Genomics (CNRGH), CEA- Institute for Biology Francois Jacob, France

Streamlining the genotyping service at the Sanger Institute using Multiplex PCR and NGS

Naomi Park, Senior Staff Scientist, Wellcome Trust Sanger Institute, UK

Flexible, high-throughput qPCR for gene expression and genotyping analysis

Fracois-Xavier Sicot, Senior Product Manager, Takara Bio Europe

Non-invasive prenatal diagnosis of monogenic disorders using droplet digital PCR

Juliette Nectoux, Molecular Geneticist, Cochin Hospital, Paris, France

Microfluidics: Where From Here?

George Whitesides, University Professor, Department of Chemistry & Chemical Biology, Harvard University

Applying High Speed Imaging Technology in Microfluidics

Nicolas Long, Global VAR/OEM Manager, Vision Research

Shaped Paper Pumps for Microfluidic Devices

Glenn Walker, Associate Professor, University of North Carolina-Chapel Hill and North Carolina State University

Acoustic Microfluidics

James Friend, Professor, Center for Medical Devices and Instrumentation, University of California San Diego

Liquid Marble Based Digital Microfluidics: Fundamental Physics and Applications

Nam-Trung Nguyen, Professor and Director of Queensland Micro- and Nanotechnology Centre, Griffith University, Australia

Microfluidics: Where From Here?

George Whitesides, University Professor, Department of Chemistry & Chemical Biology, Harvard University

Applying High Speed Imaging Technology in Microfluidics

Nicolas Long, Global VAR/OEM Manager, Vision Research

Shaped Paper Pumps for Microfluidic Devices

Glenn Walker, Associate Professor, University of North Carolina-Chapel Hill and North Carolina State University

Acoustic Microfluidics

James Friend, Professor, Center for Medical Devices and Instrumentation, University of California San Diego

Liquid Marble Based Digital Microfluidics: Fundamental Physics and Applications

Nam-Trung Nguyen, Professor and Director of Queensland Micro- and Nanotechnology Centre, Griffith University, Australia

Reports and Investor Spreadsheets

The Big List Of Quantitative PCR & Digital PCR Companies & Investors

New Developments in Liquid Biopsy Technology: Applications in PCR, qPCR, and ddPCR

The rise of microfluidic technology: inspiring multi-disciplinary innovation and collaboration

The Big List Of Microfluidics Companies & Investors

The rise of microfluidic technology: inspiring multi-disciplinary innovation and collaboration

The Big List Of Microfluidics Companies & Investors

Poster Presentations

Quantitative Phase Imaging of Cells in Flow

Silvia Ceballos, Han Sang Park, Will J. Eldridge and Adam Wax

Low Energy Extreme PCR: developing an efficient, low cost thermocycling engine

Justin Buckland, Tom Jellicoe TTP plc

A cell-direct method for rapid and robust RNA quantitation

Guoping Ren, Yan Xu, Bo Yan, and Nicole M. Nichols New England Biolabs,

Cell-free DNA enrichment for rapid and lower-cost molecular diagnostics for tuberculosis using isotachophoresis

Nobuyuki Futai, Ashwin Ramachandran, Catherine Hogan, Kanagavel Murugesan, Niaz Banaei, Juan G. Santiago

Novel XNA Molecular Clamp Application in NGS Diagnostic Platform OptiSeqTM Cancer Panels

Tao Zhang, Wenjing Feng, Michael J. Powell, Ping Wu§, Tim Tran§, Ke Zhan and Michael Sha DiaCarta Inc, USA

Development and Validation of ColoScapeTM – A New Non-Invasive Colorectal Cancer Mutation Detection Assay

Michael J. Powell, Qing Sun, Larry Pastor, Jinwei Du, Jyoti Phatak, Aiguo Zhang, Kamila Koprowski, Sir Walter Bodmer and Michael Sha, DiaCarta Inc, USA

RT-PCR Controlled by Mirror-Image L-DNA for Detecting RNA Viruses with No Sample Preparation

Nicholas M. Adams, Erin M. Euliano, Anna E. Wolfe, Christia Victoriano, Austin N. Hardcastle, William E. Gabella, Frederick R. Haselton, Biomedical Engineering, Physics and Astronomy, Chemistry, Vanderbilt University, USA

Implementation of NAT activities for viral detection in Sanofi Vitry site

S. Dos Santos, E. Lespinasse, S. Basmaciogullari, M. Marchand

Self Similar Interfacial Impedance of Electrodes in High Conductivity Media

Anil Koklu, Amin Mansoorifar, Jason Guiliani, Carlos Monton, Ali Beskok, Department of Mechanical Engineering, Southern Methodist University, Department of Physics and Astronomy, University of Texas

Impedance Based Microfluidic Device for Measuring Drug Response of Cancer Cells

Amin Mansoorifar, Anil Koklu, Shihong Ma, Ganesh V. Raj, Ali Beskok

High spatio-temporal resolved light-driven merging of microdroplets in microfluidic chambers

Zain Hayat, Abdel El Abed, Quantum and Molecular Laboratory, Universite, Paris Saclay

Self Similar Interfacial Impedance of Electrodes in High Conductivity Media

Anil Koklu, Amin Mansoorifar, Jason Guiliani, Carlos Monton, Ali Beskok, Department of Mechanical Engineering, Southern Methodist University, Department of Physics and Astronomy, University of Texas

Impedance Based Microfluidic Device for Measuring Drug Response of Cancer Cells

Amin Mansoorifar, Anil Koklu, Shihong Ma, Ganesh V. Raj, Ali Beskok

High spatio-temporal resolved light-driven merging of microdroplets in microfluidic chambers

Zain Hayat, Abdel El Abed, Quantum and Molecular Laboratory, Universite, Paris Saclay

Presentation Slides

High-Resolution Melting for improved dPCR quantification and rapid pathogen identification

Stephanie I. Fraley, Ph.D.

Liquid Biopsy for Easy and Difficult Cancers

Tim Aitman

Metastasis-Competent Circulating Tumor Cells in Colon Cancer

Dr Catherine Alix-Panabières

Evaluation of the reliability of droplet digital PCR and RT-ddPCR on a large number of samples and targets

Guillaume Pavlovic, PhD

Screening HIV-1 Vaccine and Cure-Therapy Candidates by Profiling CD4 T Cell Viral Nucleic Acids

Dr. Catherine N Kibirige

Two-Tailed PCR for Precision Diagnostics

Kennet Mattsson, standing in for Mikael Kubista, Department of Biotechnology, CAS and TATAA Biocenter

Development and Clinical Validation of Liquid ddPCR Tests for Actionable Somatic Mutations in NSCLC

Gary Pestano, Vice President, Development and Operations, Biodesix

Circulating Tumor DNA Detection from Heparinized Plasma Samples by Droplet Digital PCR

Nasrin Sarafan-Vasseur, Liquid Biopsy Scientific Leader, Research Team on Oncology (IRON), INSERM and University of Rouen, France

Challenges and Opportunities for Digital PCR in the CLIA Laboratory of the Moffitt Cancer Experience

Anthony Magliocco, Chair of Anatomical Pathology, Moffitt Cancer Center, USA

Prospects for Digital PCR in Absolute Quantification of DNA and RNA

Ward De Spiegelaere, Assistant Professor, Ghent University, Belgium

Admix™: Custom lyophilised RT-PCR reagents for point-of-use applications

Martin A. Lee, CEO Fluorogenics Limited

Explaining Biocide Tolerance of Gram Negative Bacteria

Lucy Bock, Senior Scientist/Project Team Leader, Technology Development Group, Public Health England, UK

Optimized Design of Broadly Detecting qPCR Primers and Probes Using a Conservation and Hybridization Prediction Algorithm

Jonas Blomberg, Emeritus Professor of Clinical Virology, Uppsala University, Sweden

MelTree: A Novel Workflow for the Automated Identification of a Large Number of Variants through HRM

Jean-Christophe Avarre, Head of the High Throughput qPCR Platform and Research Group Leader, University of Montpellier, France

Discordance Between Replicate qPCR Reactions

Jan Ruijter, Assistant Professor, University of Amsterdam, The Netherlands

Clinical Utility of Droplet Digital PCR on Liquid Biopsies from Patients with Castration Resistant Prostate Cancer

Daniel Wetterskog, Senior Scientist, Institute of Cancer Research, UK

High Through-Put DNA Methylation Analysis of Lung Cancer: Plasma cfDNA for Biomarker Development

Andreas Weinhaeusel, Ram Vinay Pandev, Walter Pulverer, AIT Austrian Institute of Technology, Vienna, Austria

Development, Commercialisation and On-market Improvement to Liquid Diagnostic Tests in Lung Cancer

Hestia Mellert, PhD Director, Development, Biodesix

melTree: A novel workflow for the automated identification of a large number of variants using high resolution melting

Jean-Christophe Avarre, Institute of Evolutionary Sciences of Montpellier (ISEM)

Pan-lyssavirus real-time RT-PCR assay for rabies diagnosis

Yu Li PhD Lead, Molecular Diagnostics & Genomics Team Poxvirus and Rabies Branch

Is precise partition volume (monodispersity) required for good results in dPCR? Actually, no!

Joel Tellinghuisen, Department of Chemistry Vanderbilt University Nashville

Data Analysis Pipelines and Tools for dPCR Experiments

Olivier Thas, Center for Statistics, Hasselt University, Belgium

Extraordinarily Sensitive, Multiplex PCR Assays that Assess the Abundance of Rare Mutations Associated with Cancer

Fred Russell Kramer, Diana Vargas-Gold, Salvatore A.E. Marras, Tova Gerber, Sanjay Tyagi, Public Health Research Institute, New Jersey Medical School, Rutgers University

Digital PCR for nucleic acid quantification

HENDRIK EMONS, Head of ‘Food and Feed Compliance’ Unit, European Commission’s Joint Research Centre, Health, Consumers & Reference Materials Directorate, Belgium

Two-tailed PCR for Precision Diagnostics

Mikael Kubista, Professor, Czech Academy of Sciences; Chairman of the Board, and Founder, TATAA Biocenter, Sweden

Streamlining the genotyping service at the Sanger Institute using Multiplex PCR and NGS

Naomi Park, Senior Staff Scientist, Wellcome Trust Sanger Institute, UK

Flexible, high-throughput qPCR for gene expression and genotyping analysis

Francois-Xavier Sicot, Senior Product Manager, Takara Bio Europe

Selfie-dPCR: Absolute measurement of gene expression and replication

Petar Podlesniy, Scientific Researcher, CiberNed, IIBB, Barcelona, Spain

Challenges in establishing a PCR assay for the diagnosis of invasive aspergillosis

Stephane Bretagne, Professor of Medicine, Paris Diderot University; Chair of Parasitology and Mycology, Saint Louis University Hospital; Deputy Director, French National Reference Centre for Invasive Mycoses and Antifungals, Institut Pasteur, Paris, France

Novel digital PCR and mutation enrichment technologies for the analysis of clinically relevant DNA alterations in liquid biopsies

Mike Makrigiorgos, Professor of Radiation Oncology, Dana Farber Cancer Institute and Harvard Medical School, USA

Identification of rare mutations and DNA methylation patterns in cell-free DNA using multiplexed Enhanced-ice-COLD-PCR

Jörg Tost, Director of the Laboratory for Epigenetics and Environment, National Center for Research in Human Genomics (CNRGH), CEA- Institute for Biology Francois Jacob, France

MSRE-qPCR for multiplexed analysis of DNA methylation can be accurately used for detection and validation of colorectal cancer-specific biomarkers in liquid biopsy samples

Kristi Kruusmaa, Head of Research, Universal DX Diagnostics, Spain

Non-invasive prenatal diagnosis of monogenic disorders using droplet digital PCR

Juliette Nectoux, Molecular Geneticist, Cochin Hospital, Paris, France

Bioanalysis with leaky waveguides and moving magnets

Nicole Pamme, Professor for Analytical Chemistry, University of Hull

Microfluidic solutions for cell and tissue studies

Winnie Svendsen, Associate Professor, DTU Nanotech Denmark

Affinity-Based Optical DNA Mapping In Nanochannels

Fredrik Westerlund, Associate Professor, Chemical Biology, Chalmers University

Development and Manufacturing of Fully Integrated Sample-to-Result Microfluidic Devices

Holger Becker, Co-founder and CSO, microfluidic ChipShop GmbH

Microfluidic Devices for Cell Capture and DNA Amplification

Harold Craighead, Professor of Applied and Engineering Physics, Cornell University

Highly multiplexed, two-stage isothermal assay (RAMP) for Molecular Detection at the Point of Care

Haim H Bau, Professor, Mechanical Engineering and Applied Mechanics (MEAM), University of Pennsylvania

Catalyzing Commercialization at the National Science Foundation

Rajesh Mehta, Program Director, National Science Foundation

Microfluidic Systems for Recapitulating Tumor-Immune Cell Interactions

Jeffrey T. Borenstein, Technical Director, Biomedical Engineering Center, Draper

Shaped paper pumps for microfluidic devices

Glenn Walker, Associate Professor, University of North Carolina, Chapel Hill and North Carolina State University

The Complexity Challenge: Development and Manufacturing of Fully Integrated Sample-to-Result Microfluidic Devices

Holger Becker, Co-founder and CSO, microfluidic ChipShop GmbH

Applying high speed imaging technology in Microfluidics – an overview

Nicolas Long, Global VAR/OEM Manager, Vision Research, an Ametek Company

Microfabricated Platforms for Microbiome Culture: From Artificial Mouths to Guts

Dr Todd Thorsen, Technical Staff, MIT Lincoln Laboratory

Organism-on-a-Chip Models for Neurobehavioral Screening of Disease

Pouya Rezai, PhD, PEng Associate Professor Department of Mechanical Engineering York University, Toronto, ON, Canada

Advancing Medical Device Innovation with Microfluidics

Suvajyoti Guha, PhD, Division of Applied Mechanics Office of Science & Engineering Laboratories, Center for Devices & Radiological Health, Food & Drug Administration (FDA)

Liquid marble based digital microfluidics: fundamental physics and applications

Nam-Trung Nguyen, Professor and Director of Queensland Micro- and Nanotechnology Centre, Griffith University, Australia

Droplet microfludics for single cell biology research

Rapolas Zilionis, Vilnius University, Lithuania

Commercialization of Microfluidic Devices for point-of-care applications

Vincent Linder, Ph. D