Case Study: High-throughput SNP Screening Downstream of CRISPR/Cas9 Gene Editing
Posted 31st October 2018 by Jane Williams
One of the most powerful applications of genome editing is the introduction of nucleotide substitutions in specific genomic sites. This can be used to mimic single-nucleotide polymorphisms (SNPs) or to generate stop codons that yield precise gene knockouts. However, screening hundreds of clones for a single edited nucleotide remains a challenge, especially in the absence of a corresponding phenotype.