Posted 30th April 2021 by Nicholas Noakes
Professor Andrew Martin, UCL, speaking at the 4th Global Pharma R&D AI, Data Science and Informatics Summit, described using the abYsis database and workbench for drug discovery. He showed how it is possible to explore an annotate antibody sequence and structure, including comparisons with observed residue distributions. The database can also aid with humanization by making sequences more human and library design.
Posted 19th June 2019 by Joshua Sewell
During my eight years at the Max Planck Institute for Molecular Genetics in Berlin, I led a protein technologies group. We developed a protein expression library, and then high-content protein arrays. In total, we made arrays with 10,000 different human proteins.
Posted 10th June 2019 by Joshua Sewell
The combination of simplicity and power is turning flow cytometry into the highest throughput protein analysis method yet developed.
Large-scale protein analysis, or proteomics, is still a relatively small discipline where the research front is driven by a few labs with unrestricted access to mass spectrometry (MS). MS is equivalent to a mainframe computer: very big, very sophisticated, and only a few people have the skills to use them.
Posted 7th June 2019 by Joshua Sewell
One of the frustrations I have with Flow Cytometry is when companies present their amazing new findings at conferences, and it’s quite often about TMB cells. In my case, I work on these cells perhaps 20% of the time. The rest of the time I work on cells from other parts of the human body – bone marrow, lung, bronchoalveolar lavage, spleen – and in diverse animals such as mice, rats, and even sparrow, chicken, and mosquito.
Posted 11th October 2017 by Jane Williams
An extensively used technique for evaluating the expression of tissue antigens and their behaviour is known as immunohistochemistry (IHC). In this technique, the primary antibody raised against the specific antigen and a secondary antibody against the primary one is used for detecting specific targets of clinical relevance. IHC has the greatest impact on diagnostic pathology compared to any other technique. Although modern methods of flow cytometry and molecular diagnosis have also contributed enormously, IHC still remains the most relevant solution for various pathological diagnostic problems.