Multiplex Super-Selective PCR Assays for the Detection and Quantitation of Rare Somatic Mutations
Posted 22nd February 2021 by Nicholas Noakes
Professor Fred Kramer spoke at the recent Research & Technology Series exploring Flow Cytometry / qPCR & Digital PCR / Liquid Biopsies. During his presentation, he explained how Super Selective primers enable the simultaneous identification and quantitation of rare somatic mutations in routine multiplex PCR assays, while virtually eliminating signals from abundant closely related wild-type sequences.
CE and HPLC applications for rAAV characterisation
Posted 17th January 2020 by Joshua Sewell
Despite recent advances in the gene therapy field, significant challenges lie ahead for the consistent manufacturing and release of these medicines. Analytical methods and automation need to keep pace as more medicines move towards the market and more quality control resources are required. Well-developed methods on robust platforms will be crucial to ensure the quality of these novel medicines for the doctors and patients who need them.
Rabies diagnostics with RT-PCR assays, Liquid Diagnostics and What the Future Holds for Digital PCR
Posted 16th November 2018 by Jane Williams
With its reduction in cost, and an increased number of real-time PCR users purchasing Digital PCR, the qPCR and Digital PCR market is predicted to grow to $4.94 billion by 2021. At the 4BIO event in San Francisco, three dedicated tracks proved a timely opportunity to learn first-hand about dPCR whilst keeping up-to-date with latest market developments and strategies. If you weren’t able to be there in person, these slides are now available from Hestia Mellert, Olivier Thas and Yu Li.
Digital-Droplet PCR Gets Another ‘D’
Posted 2nd November 2018 by Jane Williams
Triple-d PCR enhances digital PCR sensitivity and precision for liquid biopsies
Digital-droplet PCR (ddPCR) has been implemented in diverse fields such as cancer biomarkers, viral load detection, prenatal screening, organ donor rejection, or library assessment for next generation sequencing. Detection of emerging resistance or minimal residual disease via ddPCR in liquid biopsies is also growing rapidly.
Busting Common Myths about Quantitative PCR & Digital PCR
Posted 4th June 2018 by Jane Williams
qPCR and dPCR are mature analytical methods for quantifying selected genetic materials in a sample. Both utilise the PCR method to amplify amounts as small as a single molecular fragment (template) to levels where they can be easily detected, usually by fluorescence techniques.
The Big List of Quantitative PCR & Digital PCR Companies & Investors
Posted 30th May 2018 by Jane Williams
The global qPCR & dPCR instrumentation market is growing and is expected to reach 1631.09 million USD by the end of 2025[1].
International Women’s Day: An Interview with Patricia Silveyra
Posted 5th March 2018 by Jane Williams
In celebration of International Women’s Day on the 8th March, Global Engage is pleased to present a series of blog posts this week, showcasing the work of several key women in the field of science. The theme for this years’ International Women’s Day is ‘Press for Progress’ so we caught up with Patricia Silveyra, a full-time Assistant Professor at the Pennsylvania State University College of Medicine, to discuss what she thinks needs to be done to achieve gender equality for women in science.