Multiplex Super-Selective PCR Assays for the Detection and Quantitation of Rare Somatic Mutations
Posted 22nd February 2021 by Nicholas Noakes
Professor Fred Kramer spoke at the recent Research & Technology Series exploring Flow Cytometry / qPCR & Digital PCR / Liquid Biopsies. During his presentation, he explained how Super Selective primers enable the simultaneous identification and quantitation of rare somatic mutations in routine multiplex PCR assays, while virtually eliminating signals from abundant closely related wild-type sequences.
Multiplex and high fidelity: the potential of qPCR
Posted 28th August 2020 by Joshua Sewell
Ahead of the qPCR & Digital PCR Congress, we sat down with David Zhang to talk about his work with PCR as a diagnostic platform.
CE and HPLC applications for rAAV characterisation
Posted 17th January 2020 by Joshua Sewell
Despite recent advances in the gene therapy field, significant challenges lie ahead for the consistent manufacturing and release of these medicines. Analytical methods and automation need to keep pace as more medicines move towards the market and more quality control resources are required. Well-developed methods on robust platforms will be crucial to ensure the quality of these novel medicines for the doctors and patients who need them.
Gut Microbiome analysis – Getting it right
Posted 8th March 2019 by Joshua Sewell
The cascade of new discoveries relating health and disease to our gut microbiome has spurred the notion that we now find ourselves in the middle of a “microbiome revolution”. Just to mention some recent examples, mechanisms have been demonstrated for gut bacteria contributing to Parkinson’s disease, determining response to immune checkpoint inhibitor cancer therapy, and even autistic behavior when fecal material from autistic children was transplanted into mice.
Nucleic Acid Quantification, Mutation Enrichment Technologies & Streamlining Genotyping
Posted 30th January 2019 by Jane Williams
In just two years’ time, the digital PCR and qPCR market is set to be worth an estimated $4.94 billion. Dedicated sessions at the 4BIO Summit covered topics such as gene expression analysis, liquid biopsies and high-throughput screening. We have made the following presentation slides from Hendrik Emons, Naomi Park & Mike Makrigiorgos available.
Rabies diagnostics with RT-PCR assays, Liquid Diagnostics and What the Future Holds for Digital PCR
Posted 16th November 2018 by Jane Williams
With its reduction in cost, and an increased number of real-time PCR users purchasing Digital PCR, the qPCR and Digital PCR market is predicted to grow to $4.94 billion by 2021. At the 4BIO event in San Francisco, three dedicated tracks proved a timely opportunity to learn first-hand about dPCR whilst keeping up-to-date with latest market developments and strategies. If you weren’t able to be there in person, these slides are now available from Hestia Mellert, Olivier Thas and Yu Li.
Busting Common Myths about Quantitative PCR & Digital PCR
Posted 4th June 2018 by Jane Williams
qPCR and dPCR are mature analytical methods for quantifying selected genetic materials in a sample. Both utilise the PCR method to amplify amounts as small as a single molecular fragment (template) to levels where they can be easily detected, usually by fluorescence techniques.
The Big List of Quantitative PCR & Digital PCR Companies & Investors
Posted 30th May 2018 by Jane Williams
The global qPCR & dPCR instrumentation market is growing and is expected to reach 1631.09 million USD by the end of 2025.